Journal: Drug Design, Development and Therapy
Article Title: Astragaloside IV Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells via Activating PI3K/AKT/eNOS/NO Signaling Pathway: In vitro and in vivo Study
doi: 10.2147/DDDT.S514682
Figure Lengend Snippet: Cultivation and identification of PDLSCs. ( A ) Morphology of PDLSCs. Scale Bar: 200 μm. ( B ) Lipid droplets of PDLSCs after adipogenic induction. Scale bar: 200 μm. ( C ) Mineralized nodules of PDLSCs after osteogenic induction. Scale bar: 500 μm. ( D ) Flow cytometric analyses of PDLSCs. Mesenchymal stem cell markers (CD44, CD105) were positive and hematopoietic stem cell markers (CD34, CD45) were negative in PDLSCs.
Article Snippet: Cells were seeded in 6-well plates at a density of 1×10 5 cells per well and incubated with osteogenic induction medium (MEM with 10% FBS, 50μg/mL ascorbic acid, 0.01μM dexamethasone, and 10mM β-glycerophosphate, Solarbio, Beijing, China) or adipogenic induction medium (MEM with 10% FBS, 500mM isobutyl-methylxanthine, 10 mM insulin, 60mM indomethacin, and 0.5 M hydrocortisone, Solarbio, Beijing, China).
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